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High Speed Imaging System

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RedShirtImaging offers state of the are imaging systems for recording optical signals from biological preparations.
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RedShirtImaging offers state of the are imaging systems for recording optical signals from biological preparations. Our high speed, low noise, CMOS and CCD cameras can be used to imaging signals from single molecules to neural networks to a whole heart. They have been extensively to image voltage, calcium, TIRF and intrinsic imaging signals from individual neurons, brain slices and in vivo preparations.

The design of these imaging systems has benefited from customer suggestions and input from experienced scientists who are members of RedShirtImaging. Our turnkey optical recording systems include optics to couple light from the microscope/macroscope to the camera, the camera, computer and software.
 

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  • High Speed:

   >200fps NDR, 100fps CDS at 2048x2048

   >640fps NDR, 320fps CDS at 1024x1024-bin2

   >2,000fps NDR, 1,000fps CDS at 1024x320-bin2 - 2500fps NDR

   >1000fps CDS at 2048x180 & more

  • On-chip Bin: Unique true on-chip binning with 15Ke- well depth and 65Ke- at 2X2 binned
  • Low Noise: 2.8e- read noise without pixel correction
  • High QE: 65% without distorting micro- lenses (15um pixel)
  • Monotonic: Single A-D per channel, no dual-A-D stitching
  • NDR Mode: Double the maximum speed with over-sampling that offers significant advantages for post-analysis
     

 

Piao HH, Rajakumar D, Kang BE, Kim EH, Baker BJ. (2015) Combinatorial Mutagenesis of the Voltage-Sensing Domain Enables the Optical Resolution of Action Potentials Firing at 60 Hz by a Genetically Encoded Fluorescent Sensor of Membrane Potential. J Neurosci. 35(1):372-85.
Wright NJ, Sides LJ, Walling K. (2015) Initial studies on the direct and modulatory effects of nitric oxide on an identified central Helix aspersa neuron. Invert Neurosci. 15(1):175.

Sedmera D, Reckova M, Rosengarten C, Torres MI, Gourdie RG, Thompson RP. (2015) Optical Mapping of Electrical Activation in the Developing Heart. Microscopy and Microanalysis 11(3): 209-215.
Qian T, Chen R, Nakamura M, Furukawa T, Kumada T, Akita T, Kilb W, Luhmann HJ, Nakahara D, Fukuda A. (2014) Activity-dependent endogenous taurine release facilitates excitatory neurotransmission in the neocortical marginal zone of neonatal rats. Front Cell Neurosci. Feb 10;8:33.

Malyshev AY, Balaban PM (2014) Changes in Intracellular Calcium Ion Concentrations during Generation of High-Amplitude EPSP in Neurons in the Common Snail Neurosci Behav Physiol

Weigel S, Flisikowska T, Schnieke A, Luksch H. (2014) Hybrid voltage sensor imaging of eGFP-F expressing neurons in chicken midbrain slices. J Neurosci Methods. 233C:28-33.

Popovic MA, Gao X, Carnevale NT, Zecevic D. (2014) Cortical Dendritic Spine Heads Are Not Electrically Isolated by the Spine Neck from Membrane Potential Signals in Parent Dendrites. Cereb. Cortex 24 (2): 385-395.

Cantu D, Walker K, Andresen L, Taylor-Weiner A, Hampton D, Tesco G, Dulla CG. (2014) Traumatic Brain Injury Increases Cortical Glutamate Network Activity by Compromising GABAergic Control. Cereb. Cortex [Epub ahead of print


 

暂未实现,敬请期待
暂未实现,敬请期待

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