CULTURE MYOGRAPH SYSTEMS - 202CM/204CM
The Culture Myograph System - 202CM is designed to provide long-term functional preservation of small blood vessels by maintaining them under controlled culture conditions of intravascular pressure and flow. By using a closed, sterile system and standard culture methodology, vessels can be studied for several days.
The system has an integrated DMT microscope (with a USB CCD camera) for continuous monitoring of the vessel during the culture period. The system contains a stage with built-in heating and two reservoirs that are pressurized by an external pressure unit to maintain a consistent intravascular pressure.
During culture, all possible parameters such as lumen diameter, wall thickness and cross sectional area can be measured and recorded using our MyoVIEW. Addition of the optional FlowMeter 162FM to the system allows accurate flow measurement during culture.
The design of the small chamber allows for easy access to insert the vessel for cannulation. The cannulae are easily changed and the chamber cover allows appropriate viewing via its submerged centre. All parts of the chamber unit can easily be disassembled and sterilized by autoclaving. Two inlet/outlet ports are available for insertion of probes or superfusing medias.
The design of the small chamber allows easy access for vessel cannulation. The cannulae can easily be replaced, and the chamber cover allows appropriate viewing via its submerged centre. All parts of the chamber unit can be disassembled and sterilized by autoclaving. Two inlet/outlet ports are available for insertion of probes or superfusion of culture medium or buffer.
DMT has developed a 4-channel version of the popular Culture Myograph System - 202CM. By providing one heating unit for four stages and up to four small individual pressure units, productivity can be increased significantly. Only the essential components for vessel culture are duplicated in the 204CM model.
- Provides long-term preservation of vessels >60 μm
- System includes integrated microscope with camera for continuous vessel monitoring
- Comes with MyoVIEW to facilitate vessel monitoring during culture
- Possible to measure lumen size, wall thickness, cross-sectional area and other vascular parameters during culture
- Culture chamber easy to sterilize
"Arterial Wall Stress Controls NFAT5 Activity in Vascular Smooth Muscle Cells"
Clemens Scherer, Larissa Pfisterer, Andreas H. Wagner, Maren Hödebeck, Marco Cattaruzza, Markus Hecker, Thomas Korff